| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
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| Autores: |
Hara, Olgga A
Liao, Min
Baticados, Waren
Bannai, Hiroshi
Zhang, Guohong
Zhang, Sofa
Lee, Eung-goo
Nishikawa, Yoshifumi
Claveria, Florencia G.
Igarashi, Makoto
Nagasawa, Hideyuki
Xuan, Xuenan
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| Data: |
2006
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| Ano: |
2006
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| Palavras-chave: |
Canine
ELISA
Neospora caninum
NcGRA7
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| Resumo: |
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were positive for antibodies to N. caninum. Of the 32 ELISA-positive samples, 29 (90.62%) were confirmed as positive by Western blot analysis with whole tachyzoite antigens. These results indicate that the recombinant NcGRA7t would be a reliable antigen in ELISA for the diagnosis of canine neosporosis.
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/995
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| Editor: |
National Research Center for Protozoan Diseases
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| Formato: |
application/pdf
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| Direitos: |
National Research Center for Protozoan Diseases
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